After the outbreak of HPAI in 2015, all biosecurity activities were re-considered in the egg industry. Plastic egg flats are often used to transport eggs from off-line egg farms to central egg processing plants. In so doing, movement of the egg flats among different farms has the inherent risk of moving pathogens from farm to farm. This project investigates the protocol of increased biosecurity through heat-treating the egg flats to a certain threshold temperature (e.g., 130-140°F) for a certain period of time to ensure that any pathogens on the flats will be destroyed. This experiment will determine how fast heat can penetrate into a wrapped stacks of egg flats and determine the most effective design of a heating system so it can be installed on a farm.